Superdex Peptide 10/300 GL 货号：17517601

Superdex Peptide 10/300 GL will be discontinued end of 2017 and replaced by Superdex 30 Increase 10/300 GL during 2017. Superdex Peptide 10/300 GL is a prepacked column for high-resolution size exclusion chromatography for small-scale preparative purification and analysis of peptides and other small biomolecules with molecular weights between 100 and 7000.

Superdex 30 Increase 10/300 GL 货号:29219757

Superdex 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with Mr ~ 100 to ~ 7000, such as peptides

• Improved performance – improved resolution and runtime compared to predecessor Superdex Peptide
• Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
• Very high resolution – small bead size and narrow particle size distribution provide high resolution, for high protein purity.
• High flow rate – due to rigid beads giving excellent pressure/flow properties.

SEC columns for analysis and purification of peptides

Superdex 30 Increase is suitable for both preparative and analytical purposes. 

The fractionation range allows preparative separation of peptides, oligosaccharides and other small biomolecules (Mr ~ 100 to ~ 7000) with sample volumes up to 500 µL. 

These columns also enable protein characterization, including aggregate analysis, size homogeneity analysis, and interaction studies, where high-quality data can be achieved

Cleaning-in-place (CIP) capabilities

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different biomolecules, with minimal risk of carry-over between samples. 

Regular cleaning can also increase column lifetime.

Two SEC column sizes

Two size exclusion chromatography (SEC) column sizes are designed to serve different purposes:

• 10/300 GL column, 10 mm × 300 mm, for high resolution analysis as well as for small-scale (25–500 µL) preparative purification, such as the polishing step in a purification protocol
• 3.2/300 column, 3.2 mm × 300 mm, for high resolution analysis and purification of sample volumes of 4–50 µl, when sample amount is limited and low buffer consumption is important
• It is also possible to order Superdex 30 Increase resin to be packed in 5/150 GL column, 5 mm x 150 mm, as custom designed product.

Parameter

Superdex 30 Increase 10/300 GL

Bed dimensions    10 × 300 mm     

Bed height    300 to 310 mm     

Bed support    Filter     

Bed volume    ~ 24 mL     

Chemical stability    Conditions where the resin may be operated without significant change in function:
• Trifluoroacetic acid, up to 0.2%
• Isopropanol, up to 5%
• Dithiothreitol (DTT), up to 5 mM
• Acetonitrile, up to 70%
• Formic acid, up to 1%
• All commonly used aqueous buffers, pH 3 to 12
• Urea, up to 8 M
• Guanidine hydrochloride, up to 6 M
• Ionic and nonionic detergents, e.g., 1% SDS
• Methanol, up to 70%

Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function:
• Sodium hydroxide, up to 1 M
• Ethanol, up to 70%
• Isopropanol, up to 30%
• Hydrochloric acid, up to 0.1 M
• Trifluoroacetic acid, up to 1%
• Dimethyl sulfoxide (DMSO), up to 10%

Avoid:
• Oxidizing agents
• Unfiltered samples and eluents     

Chemical stability (column hardware)    Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.     

Column i.d.    10 mm    

Fractionation range (Mr) (globular proteins)    100 to 7000     

Material (column hardware)    Borosilicate glass, polyetheretherketone (PEEK), polypropylene (PP), ethylene propylene diene monomer (EPDM), and perfluoro-rubber (PFR)     

Material (bed support)    Polyethylene (PE) filter     

Material (column tube)    Borosilicate glass     

Matrix    Composite of cross-linked agarose and dextran     

Operating flow rate, max.    1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature     

Operating flow rate, recommended1    0.80 mL/min     

Particle size, d50V2    ~ 9 µm     

Sample volume    25 to 500 µL     

Storage conditions    4˚C to 30˚C, 20% ethanol     

Theoretical plates    > 43 000 m-1     

Typical pressure drop over the packed bed3    ~ 3 MPa     

pH stability, cleaning-in-place (CIP)    1 to 14     

pH stability, operational    3 to 12    

1、Water at 20˚C to 25˚C.

2、Median particle size of the cumulative volume distribution.

3、At 25˚C in water. Determine the individual column limit according to product instructions for use.